• TGs are an important part of the human diet.
• NARP-HPLC allows separation of triacylglycerols according to their fatty acid composition.
• APCI mass spectrometry is used for the identification of triacylglycerols.
• The NARP-HPLC/APCI-MS response factor approach is used in triacylglycerol quantitation.
• Average parameters calculated from triacylglycerol composition characterize the nutritional properties of plant oils.
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