Carbohydrate and branched chain amino acid metabolism Thiamine serves as a cofactor for several enzymes involved in carbohydrate catabolism, including pyruvate dehydrogenase, transketolase, and alpha-ketoglutarate, and for the branched-chain alpha-keto acid dehydrogenase complex that is involved in amino acid catabolism (Singleton & Martin 2001). Some of these enzymes are also important in brain oxidative metabolism (Molina et al 2002). Neurotransmitter biosynthesis Thiamine is involved in the biosynthesis of a number of cell constituents, including the neurotransmitters acetylcholine and GABA. DNA Thiamine is involved in the synthesis of precursors of DNA, therefore thiamine use is increased in tumours.

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