In vitro tests have demonstrated activity against Escherichia coii, Listeria monocytogenes and Salmonella enterica for thyme extract (Friedman et al 2002). A review of the antibacterial and antifungal properties of the essential oil of thyme in vitro has demonstrated effectiveness against a wide range of pathogens including Clostridium botulinum, E. coli, Haemophilus Influenzae, Klebsiella pneumoniae, Salmonella typhi, Staphylococcus aureus and Candida albicans (Kalemba & Kunicka 2003). Effects are most likely due to the eugenol, thymol and carvacrol constituents Aqueous thyme extract also exhibited a significant inhibitory effect on Helicobacter pylori, reducing both its growth and its potent urease activity in vitro (Tabak et al 1996).

Thymol and eugenol have demonstrated antifungal activity by establishing the ability to alter the cell wall and membrane of the yeast Saccharomyces cervisiae (Bennis etal 2004).

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