1. Riboprobe Gemini System II Buffers Kit (Promega, Southampton, UK) includes cold ribonucleotides, DTT, Transcription optimized buffer. Store at -20°C. NTPs go off, so kits to suit level of use should be bought.
2. T3 and T7 RNA polymerase (Promega, Southampton, UK). Store at -20°C.
3. DIG-UTP (Boehringer Mannheim, Lewes, UK). Store at -20°C.
4. RNasin Ribonuclease Inhibitor (Promega, Southampton, UK). Store at -20°C.
5. Restriction enzymes (single cut 5' and 3' of insert).
6. Phenol and chloroform.
7. 7.8 M Ammonium acetate. 8.4 M Lithium chloride.
Was this article helpful?