1. 0.8 pg of 32P-labeled linkers is needed for each cDNA preparation (see Note 4). An example reaction is the following:
1 |L T4 polynucleotide kinase (~5 U); 8 |L H2O. Incubate for 15 min at 37°C.
1 |L T4 polynucleotide kinase;
3. Heat-kill the enzyme at 80°C for 15 min, and then cool on ice.
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