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Some transgenic animals may be infertile owing to an inability to mate or to rear a litter. With mice, in vitro fertilization (IVF) can be used to continue the line. the technique involves superovulating females and fertilizing the eggs with sperm taken from the epididymis of males. IVF eggs are then transferred to a 0.5-d pc pseudopregnant recipient female. The strain of mouse used should be the same as those used for the original transgenic mouse production (e.g., C57BL/6J X CBA/JF1). Males should be more than 6 wk of age and preferably studs rested the previous night. Females should be at least 4 wk of age. The described protocol employs a single male and 10 females.

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Get Pregnant - Cure Infertility Naturally

Get Pregnant - Cure Infertility Naturally

Far too many people struggle to fall pregnant and conceive a child naturally. This book looks at the reasons for infertility and how using a natural, holistic approach can greatly improve your chances of conceiving a child of your own without surgery and without drugs!

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