Fate Mapping with Lipophilic Membrane Dyes

1. DiI (l,l'-dioctadecyl-3,3,3',3'-tetramethylindocarbocyanine) perchlorate, cat. no. D-282 from Molecular Probes (Eugene, OR).

2. Steromicroscope and fiber-optic light source.

3. Micromanipulator attached to base plate or directly to stereomicroscope.

4. Pressure injector and micropipet holder.

5. Micropipet puller.

6. 9-V battery and microelectrode holder.

From: Methods in Molecular Biology, Vol. 97: Molecular Embryology: Methods and Protocols Edited by: P. T. Sharpe and I. Mason © Humana Press Inc., Totowa, NJ

8. DABCO (and antiquenching agent from Sigma, St. Louis, MO).

9. Paraformaldehyde.

10. Dimethyl formamide (DMF).

11. Dimethyl sulfoxide (DMSO).

12. 1 M lithium chloride.

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