Demonstration of Embryonic Induction by Transplantation of Hensens Node 21 Materials

1. Dissecting microscope, preferrably one with trasmitted light base.

2. Pannett-Compton saline: solution A: 121 g NaCl, 15.5 g KCl, 10.42 g CaCl22H2O, 12.7 g MgCl26H2O, H2O to 1 L; solution B: 2.365 g Na2HPO42H2O, 0.188 g NaH2PO42H2O, H2O to 1 L; before use, mix (in order): 120 mL A, 2700 mL H2O, and 180 mL B.

3. Two pairs of watchmakers' forceps, number 4 or 5.

4. One pair of coarse forceps, about 15 cm (6 in.) long.

5. One pair of small, fine scissors, with straight blades about 2 cm (3/4 in.) long.

6. A spoon/spatula or teaspoon.

7. Petri dish (about 10-15 cm diameter) to collect embryos.

8. Container for egg waste.

9. Pasteur pipet with the end cut off at the shoulder, stump flamed to remove sharp edges, and rubber teat.

10. Pasteur pipet (short form), end lightly flamed to remove sharp edges; rubber teat.

11. Pyrex baking dish about 2 in. (5 cm) deep, 2 L capacity.

12. 35-mm Plastic dishes with lids.

13. Watch glasses, about 5-7 cm diameter.

14. Rings cut from glass tubing, approx 27 mm outer diameter, 24 mm inner diameter, 3-4 mm deep.

15. Very fine needles (e.g., entomological size A1 or D1) or sharpened tungsten wire mounted by melting the fine end of a Pasteur pipet (to act as a handle) or into a metal needle holder.

17. Plastic lunch box with lid for incubating culture dishes.

18. 38°C Incubator.

19. Hens' eggs incubated 12-18 h (depending on stage needed).

20. Quails' eggs incubated 12-18 h (depending on stage needed).

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