Collection and Concentration of Viral Stocks

1. 10-cm Tissue-culture dishes.

2. Sterile 50-mL plastic centrifuge tubes.

3. Sterile 1.5-mL microfuge tubes.

4. 40 mL polyallomer ultracentrifuge tubes (Beckman, Fullerton, CA): sterilize by rinsing well in 70% ethanol and air-drying in the tissue-culture hood.

5. Phosphate-buffered saline (PBS). PBS tablets are available from Sigma.

7. Reduced serum CEF media (DMEM + 5% FCS + 1% CS).

Was this article helpful?

0 0

Post a comment