1. Pannett and Compton (5) buffered salt solution or Howard's Ringer, autoclaved.
2. Antibiotic/antimycotic solution (100X) (Gibco)
3. Howard's Ringer: 7.2 g NaCl, 0.23 g CaCl2H2O, and 0.37 g KCl. Dissolve in 1000 mL double-distilled water. Adjust to pH 7.2 with one or two drops of 0.5 M
Fig. 2. Cartoon of an egg yolk being cut around the equator to remove the vitelline membrane.
NaOH. Fill in suitable bottles or flasks, autoclave the day before, and keep refrigerated.
4. Pannett and Compton solution:
a. Solution A: 12.11 g NaCl, 1.55 g KCl, 0.77 g CaCl, and 1.27 g MgCl26H2O. Dissolve in 100 mL double-distilled water and autoclave. Make up multiples if required in large amounts, keep in aliquots of 40 mL, and autoclave.
b. Solution B: 0.189 g Na2HPO42H2O and 0.019 g NaH2PO42H2O. Dissolve in 100 mL double-distilled water and autoclave. Make up multiples if required in large amounts, keep in aliquots of 60 mL, and autoclave.
c. Working solution: To make up working solution, autoclave 900 mL double-distilled water with 0.9 g glucose in a 1000-mL Erlenmeyer flask the day before. When cool, add 40 mL of solution A and 60 mL of solution B in that order, and keep at 4°C overnight. Working solution can be kept at 4°C for several days if not used up on the day of transplantation.
Was this article helpful?