Methods available for the estimation of total trans unsaturation and to determine individual trans fatty acids are outlined in Table 1. At present there is no one simple and accurate method suitable for both research applications and for use in the food industry. In dietary studies data for trans fatty acid intake are generally expressed as the sum of the fatty acids containing trans double bonds, and there is generally no differentiation between the different isomers.
A report from the British Nutrition Foundation (BNF) in 1995 highlighted concerns over the variations in estimations of trans fatty acid concentrations in some food products provided by different analytical techniques. A thorough review of the available analytical techniques was called for.
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