Sms Modulates Cellular Antioxidant Potential Through Induction Of

GPX is an enzyme participating in the capture of H2O2 and hydroperoxides produced in the cell. Therefore, the change in the activity reflects more or less overall tissue damage or cellular potential of antioxidant defense. Thus the preventive effect of SMS on GPX activity loss was further studied by the immunohistchemical method in culture cells using anti-GPX monoclonal antibody conjugated with FITC fluoroprobe. As shown in Figure 4, GPX activity was significantly induced by incubating cells with SMS prior to H2O2 stress, although H2O2 also induced GPX, as was reported elsewhere (13). This SMS-dependent enhancement of GPX activity was also confirmed by RT-

Figure 4 SMS and H2O2-dependent GPX stain for GPX.

expression of GPX. PI stain for nucleus; a-

Figure 4 SMS and H2O2-dependent GPX stain for GPX.

expression of GPX. PI stain for nucleus; a-

PCR at the transcriptional level (unpublished data), indicating that the protective action of SMS against oxidative abuse in the brain is due not only to its strong hydroxyl-radical-scavenging activity but also to its role as cell functional modulator.

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