Materials Used In Embryo Culture

Appropriate embryo tested culture dishes. 3. Fertilization, cleavage, and blastocyst media. Comparison of sequential media and single-step medium culture protocols Factors Sequential media Single-step medium Comparison of sequential media and single-step medium culture protocols Require change of medium around D3 of culture Can be used for complete preimplanta-tion period with no medium change Blastocyst medium does not contain EDTA that may reduce glycolysis Contains EDTA that may compromise...

Introduction Of Embryo Culture

Current IVF laboratory practice is to supplement all commercial IVF embryo culture media with some protein. Actually, most if not all mainstream commercial media manufacturers will provide Gary D. Smith et al. eds. , Embryo Culture Methods and Protocols, Methods in Molecular Biology, vol. 912, DOI 10.1007 978-1-61779-971-6_8, Springer Science Business Media, LLC 2012 complete or ready to use protein-supplemented products. It may be prudent to consider the history and origins of culture media...