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p is the overall average antimicrobial activity for the whole series, and a, b and c and contributions of the groups -R, -X and -Y, respectively. The identity of the terms prefixed

Figure 5.5 Biphasic relationship fitted better by bilinear equation than by a parabola.

by the letters a, b and c can best be explained if it is imagined that the contributions to the total antimicrobial activities made by the groups in position R can be determined experimentally, and are given in column 4 of Table 5.7. a[H] will then be defined by Free-Wilson analysis as the mean of the figures in column 4 involving R=H, minus the means of all the figures in column 4, i.e.

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