Info

PROTEIN AND AMINO ACIDS

E 20

Protein Requirement (g/kg/day)

FIGURE 10-5 Distribution of the estimated protein requirements for 225 individuals (Rand et al., 2003) in a trimmed data set showing the skewness of protein requirement.

SOURCE: William Rand, personal communication, 2002.

The median requirement, potentially of use as the EAR, was calculated in two different ways: first as the median of the entire sample of 235 individuals in the primary data set (weighting all individuals the same), and second as the median of the medians of each distinct substudy (weighting each substudy equally) (Rand et al., 2003). In either case, data from all individuals were included in the analysis.

The results of these analyses are included in Table 10-12. Because of the non-normality of the individual data, nonparametric tests were used (Mann-Whitney and Kruskal-Wallis) to compare requirements between the age, gender, diet, and climate subgroups (Table 10-13). Where nonsignificant differences were found, Analysis of Variance was used for power calculations to roughly estimate the differences that could have been found with the data and variability. Separate analyses were conducted for all the

TABLE 10-12 Analyses of Linear Regression Analysis of Nitrogen Requirements in Adults

Linear Regression—

3^

Median Nitrogen

Requirement

(mg/kg/d)

Linear

Q)

Regression— Slope

Intake

(mg N/kg/d)

O"

Data Sets Used

Men Women

Q.

19 Estimation studies (n = 235; men = 181, women = 54)

109 91

105

0.47

-48

SU (D

95% confidence intervals

(101, 110)

(0.44, 0.50)

(-51, -45)

-n

32 substudies (n = 32 group medians;

102 102

102

0.49

-47

CT (D

men = 24, women = 8)

-n

All individual data (n = 1,593)

103

Q)

Linear regression model (grouped data)

122

S.

Quadratic regression model (grouped data)

114

Asymptotic growth exponential (grouped data)

116

> o

Asymptote

42

al tj)

Linear bi-phase

108

o

Breakpoint

126

IT O

Asymptote

-7.2

(D <3.

PROTEIN AND AMINO ACIDS 639

individual requirements and for substudies, both excluding and including secondary estimation data (Rand et al., 2003).

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