If anticariogenic effects of phytochemicals are demonstrated by some in vitro experiments as described above, an in vivo experiment using experimental animals (e.g., rats) is carried out to confirm that the test material has anticariogenic effects in the systemic body. The animal experiment to evaluate anticariogenic effects consumes much expense and time. In other method, human plaque is also used the in vivo experiment. "Touch electrode method" and "plaque sampling method" are used for the purpose of measuring human plaque pH (Frostell, 1970; Stephan, 1940). Muhlemann et al develop " indwelling plaque pH telemetry method" (Graf H, et al 1966). In this section, we show an outline on animal experiment.
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