In vitro experiments to evaluate the anticariogenic effects of phytochemicals include observations of pH decline by acid production, inhibitory effects on glucan production by GTase from mutans streptococci and sucrose-dependent cell adhesion on smooth surfaces by mutans streptococci. In addition, antibacterial effect and the evaluation of plaque accumulation or enamel demineralization by using artificial mouth also have been investigated (Hinoide et al, 1984; Pigman et al, 1952). We have evaluated the anticariogenic effects of phytochemicals based on pH decline, glucan production by GTase and sucrose-dependent cell adhesion.
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