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pH was measured after incubation for 1 h. Control: water was added instead of Alg53 (Hashiguchi-Ishiguro et al, 2009)

Dilution factors of Alg53

pH was measured after incubation for 1 h. Control: water was added instead of Alg53 (Hashiguchi-Ishiguro et al, 2009)

Fig. 5. Inhibitory effect by different concentrations of partially decomposed alginate by V. alginolyticus SUN53 on acid production from glucose by S. sobrinus 6715

Fig. 5. Inhibitory effect by different concentrations of partially decomposed alginate by V. alginolyticus SUN53 on acid production from glucose by S. sobrinus 6715

Control ELM ES Oolong Control ELM ES Oolong

ELM, extractive from the leaves of Morus alba; ES, extractive from Salacia chinensis Reaction mixture [GTase, 0.2 mL; sucrose solution, 0.31 mL (includes 14C-sucrose, 20 ^Ci); test substance, 0.1 mL] incubated at 20° for 24 h at 37°C. The final concentration of sucrose in the reaction mixture was 1%. Glucan was expressed as the relative amount (%) of glucan produced as compared with the amount produced with the negative control (distilled water) (Hashiguchi et al, 2011).

Control ELM ES Oolong Control ELM ES Oolong

ELM, extractive from the leaves of Morus alba; ES, extractive from Salacia chinensis Reaction mixture [GTase, 0.2 mL; sucrose solution, 0.31 mL (includes 14C-sucrose, 20 ^Ci); test substance, 0.1 mL] incubated at 20° for 24 h at 37°C. The final concentration of sucrose in the reaction mixture was 1%. Glucan was expressed as the relative amount (%) of glucan produced as compared with the amount produced with the negative control (distilled water) (Hashiguchi et al, 2011).

Fig. 6. Inhibitory effects of the extractive from the leaves of Morus alba, Salacia chinensis and oolong on insoluble glucan produced by GTase

(A) Water-insoluble glucan (B) Water-soluble glucan

(A) Water-insoluble glucan (B) Water-soluble glucan

Dilution factors of Alg53 Dilution factors of Alg53

Reaction mixture [3% sucrose (final concentration, 1%) in 0.1 M phosphate buffer (pH 6.8), 1 mL; GTase from S. sobrinus, 0.3 mL; 0.1 M phosphate buffer (pH 6.8), 1.4 mL; Alg53, 0.3 mL] incubated at 20° for 24 h at 37°C. Glucan was expressed as the relative amount (%) of glucan produced as compared with the amount produced in the absence of Alg53. The amount of total carbohydrate was measured at 490 nm by the phenol-sulfuric acid method. Dates are expressed as mean values of duplicate assays (Hashiguchi-Ishiguro et al, 2009).

Dilution factors of Alg53 Dilution factors of Alg53

Reaction mixture [3% sucrose (final concentration, 1%) in 0.1 M phosphate buffer (pH 6.8), 1 mL; GTase from S. sobrinus, 0.3 mL; 0.1 M phosphate buffer (pH 6.8), 1.4 mL; Alg53, 0.3 mL] incubated at 20° for 24 h at 37°C. Glucan was expressed as the relative amount (%) of glucan produced as compared with the amount produced in the absence of Alg53. The amount of total carbohydrate was measured at 490 nm by the phenol-sulfuric acid method. Dates are expressed as mean values of duplicate assays (Hashiguchi-Ishiguro et al, 2009).

Fig. 7. Inhibitory effect of partially decomposed alginate by V. alginolyticus SUN53 on water-insoluble and water-soluble glucan produced by GTase from S. sobrinus

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