Demineralization protocol

Teeth were exposed to a pH cycling system to develop caries-like lesions. Each day teeth were incubated in demineralization solution (lactic acid and Carbopol [pH = 5.0], 50% saturated with hydroxyapatite) for eight hours, rinsed with de-ionized water and placed in artificial saliva for 30 minutes, followed by two seconds of brushing with a powerbrush (Sonicare, Philips) and fluoridated dentifrice (NaF, 1,100 ppm F), rinsed again and placed back in artificial saliva until next demineralization period (next day).

Number of

teeth with newly developed WSL

Frequency of patients

O")

Percent of total parieurs

Cumulative frequency

Cumulative percent

0

95

27,. 1

95

27.1

1

57

16.3

152

43.4

2

4S

13.7

200

57.1

3

IS

5 1

218

62.2

4

21

6.0

239

68.3

5

IS

5.1

257

73.4

6

21

2.3

265

75.7

7

11

3.1

276

78.9

8

10

2.8

286

81.7

9

9

2 6

295

84.3

10

6

1.7

301

86.0

11

5

1.4

306

87.4

12

8

2.3

314

89.7

13

6

1.7

320

91 4

14

5

1.4

325

92.9

15

7

2.0

332

94.9

16

5

1.4

337

96.3

17

4

1.1

341

97.4

18

4

11

345

98.6

19

1

0 3

346

98.9

20

2

0.6

348

99.4

21

1

0.3

349

99.7

22

1

0.3

350

100.0

Table 1. Incidence of white-spot lesions (WSLs)

Table 1. Incidence of white-spot lesions (WSLs)

Solutions were refreshed daily during the experimental period of 15 days. On day 15, all teeth were removed from the saliva solution, rinsed under tap water and stored in 100% humidity. To assess demineralization, Quantitative Light-induced Fluorescence (QLF) and Confocal Laser Scanning Microscopy (CLSM) were used. Both procedures were carried out at the Oral Health Research Institute (IU) in Indianapolis, IN.

Parameter

Standard

Variable

estimate

error

t-value

P value

Sex

-0.73

0.55

-1.33

0.19

Age group

-0.59

0.28

-2.12

0.03*

Treatment

0.07

0.02

2.76

0.01*

length

Initial oral

-0.08

0.56

-0.14

0.89

hygiene

score

Number of

1.88

0.39

4.86

<0.00*

oral hygiene

discussions

Table 2. Multivariabile regression model.

Age

Trend, fewer new lesions as age increases

Treatment length

Trend. 0.08 new lesions per month of treatment

Oral hygiene

Counter-intuitive trend, number increases as number of discussions increased

Table 3. Inferential statistics. Adjusted R-square = 0.11. This model accounts for 11% of the variation inWSL development.

Table 3. Inferential statistics. Adjusted R-square = 0.11. This model accounts for 11% of the variation inWSL development.

WSL Cavitations Restorations

Fig. 3. Distribution of patients with at least one new lesion.

0 0

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