Summary

Gene guns are a unique method of gene delivery that employs kinetic energy to overcome transfection bottlenecks to deliver chemicals, proteins, mRNA, or genes directly into cells on

Figure 9 Rapid in vivo expression screening of different introns and poly adenylation sequences by gene gun delivery into the skin. Plasmids bearing the indicated sequences driving luciferase expression were delivered into the skin of the ears of BALB/c amice by gene gun. Expression comparison was performed the next day by luciferase assay on the shot skin.

Figure 9 Rapid in vivo expression screening of different introns and poly adenylation sequences by gene gun delivery into the skin. Plasmids bearing the indicated sequences driving luciferase expression were delivered into the skin of the ears of BALB/c amice by gene gun. Expression comparison was performed the next day by luciferase assay on the shot skin.

microparticle ''bullets.'' Gene guns can transfect many tissues that are refractory to vectored gene delivery, and are also quite efficient at direct delivery of plasmids into living animals and humans. This approach allows simple plasmids to be delivered into the host that encode only the gene of interest. This avoids problematic immune responses against vector antigens that can prevent multiple administration. Although gene guns can modify both dividing and nondividing tissues in vitro and in vivo, they do not modify a massive number or percent of cells in a given transfection event. Therefore, gene guns are best applied to systems that amplify the effects of a small amount of transgene product. Gene guns therefore have great utility in the delivery of antigen or cytokine genes into the immune system to stimulate or block immune responses. Gene guns also have utility in surgical settings to repulse neurons to reduce pain and for the delivery of transgene proteins to enhance wound and burn repair.

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