Dividing And Nondividing Cellular Targets

MuLV, and the vectors derived from it, are only able to infect dividing cells. This is because the preintegration nucleoprotein complex is unable to cross an intact nuclear membrane. In contrast, the prototypical lentivirus HIV-1 has been shown capable of nuclear import even when an intact membrane exists, and HIV-1-derived lentiviral vectors are therefore able to transduce nondividing cells (76). This property of HIV vectors makes them particularly attractive candidates for gene therapy when the target cell is nondividing and stable integration of the transgene is required. However, as outlined below, the restricted tropism of MuLV for dividing cells can also be used to advantage, limiting transduction to dividing cells such as tumor cells.

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