Info

lasting expression of a desired protein either for treatment of a local disease or for research purposes. Results of these studies demonstrate that electroporation is an excellent method for delivering genes to multiple cell layers within the cornea with extremely high levels of gene expression and little, if any, inflammatory response or tissue damage (90,91). Retinal ganglion cells have also been targeted. After a first demonstration showing retrograde labeling of up to 41% of the total ganglion cells in the electroinjected area (91), other studies with brain-derived neurotrophic factor (BDNF) gene transferred by in vivo electroporation showed protection of axoto-mized retinal ganglion cells against apoptosis (91,92).

Table 3 Electroporation for Plasmid Delivery in Tumors

Transgene

Species

Endpoint

Ref. Citation

CpG (luciferase)

Mouse

Tumor volume

(86)

0 0

Post a comment