Iiretroviral Vectors

Retroviruses can efficiently carry out gene transfer to many cell types, where they stably integrate their genomes into the host cell chromosome. Gene transfer vectors derived from retroviruses therefore provide the possibility of long-term gene expression. To date, the most common vectors used in clinical gene therapy protocols have been based on the murine leukemia virus (MuLV), and various packaging systems to enclose the vector genome within viral particles have been developed [reviewed in (4)]. The vectors themselves have all the viral genes removed, are fully replication defective, and can accept up to approximately 6-8 kilobase (kb) of exogenous DNA.

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