Advantages Of Retroviral Vectors A Fully Defective Vectors

Retroviral vectors represent a truly defective vector system; none of the viral proteins need to be expressed in the target cell for efficient gene transfer to occur and can simply be provided in trans in the producer cell. In addition, no de novo viral protein synthesis is needed to maintain or repair the integrated provirus. This has implications for long-term gene expression in the transduced cells as even a low-level production of viral proteins will increase the likelihood of an unwanted immune response being triggered against the transduced cells (in addition to any immune response that may be mounted against the transgene itself). Such vector antigenicity is largely responsible for the transient nature of the gene expression seen with current adenoviral vectors, although the problem is being aggressively pursued through the development of helper-dependent or ''gutless'' adenoviral vectors.

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